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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: SNHG15 is a bifunctional MYC-regulated noncoding locus encoding a lncRNA that promotes cell proliferation, invasion and drug resistance in colorectal cancer by interacting with AIF

Fig. 5

a Schematic representation of CRISPR/Cas9 technology to delete the region between exons 3 to 5 of SNHG15 and annealing sites of primers to detect deleted and non-deleted regions on RNA. b Relative RNA level for deleted, non-deleted region and SNORA9 from wild type (no. 3) and homozygote clones (no. 10 and no. 83) with the different primer sets. c Cell proliferation, d colony formation ability after knockout of SNHG15. e Cell cycle profile and (f) Percentage of apototic cells. g Tumor formation capacity after deletion of exons 3 to 5 of SNHG15 in LoVo cells. The statistical analysis is performed by two-tailed Student’s t-test and graphs shows mean ± SEM of values (*p < 0.05, **p < 0.01, ***p < 0.001)

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