Fig. 1

Mutant K-Ras suppresses miR-199b expression in NSCLC. a Heatmap showing the 46 significantly downregulated miRNAs in K-Ras (G12D)-overexpressing NSCLC cell lines. b miR-199b expression is downregulated in specimens from NSCLC patients with K-Ras mutations (n = 24) compared to those from K-Ras wild-type specimens (n = 36). c K-Ras-mutated NSCLC cell lines have lower miR-199b expression levels than NSCLC cell lines with wild-type K-Ras. d miR-199b was significantly decreased in K-Ras-mutated NSCLC specimens compared to their respective adjacent tissues (n = 24). e No difference was observed between adjacent tissues and NSCLC tissue from K-Ras wild-type patients (n = 36). f Silencing of K-Ras increased miR-199b expression in both K-Ras-mutated NSCLC cell lines, A549 and H2122. Cells were transfected with a K-Ras shRNA expression vector or a scrambled vector for 72 h and then subjected to miR-199b analysis. g Overexpression of mutant K-Ras (G12D) inhibits miR-199b expression in both K-Ras wild-type NSCLC cell lines, H522 and H1975. Cells were transfected with a K-Ras (G12D) expression vector or an empty vector for 72 h and then subjected to miR-199b analysis