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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Restoration of mutant K-Ras repressed miR-199b inhibits K-Ras mutant non-small cell lung cancer progression

Fig. 2

Inhibition of miR-199b stimulates NSCLC progression. a-c Inhibition of miR-199b promotes cell proliferation, soft agar colony formation, and cell invasion in both H522 and H1975 NSCLC cell lines. Cells were transfected with negative control oligonucleotides (NC) or antisense oligonucleotides of miR-199b (ASO miR-199b) and were then subjected to cell proliferation, soft agar colony formation and invasion analysis. d-e Inhibition of miR-199b stimulates tumor growth and cancer cell proliferation in the H522 xenograft model. Using stably expressing miR-199b-antisense H522 cells or control cells, xenografts were generated in nude mice. One month after cell injection, mice were sacrificed, and the tumor weights were measured. Cell proliferation was measured using Ki-67 immunohistochemistry in tumor tissues. f Inhibition of miR-199b promotes NSCLC cell metastasis in vivo. Stably expressing miR-199b-antisense H522 cells or control cells were injected into nude mice by tail vein injection. The mice were sacrificed 1 month after cell injection, and the tumors on the lung were counted. g Kaplan-Meier analysis of the overall survival of patients with lung adenocarcinoma for whom overall survival information was available in the TCGA lung cancer data set

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