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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Restoration of mutant K-Ras repressed miR-199b inhibits K-Ras mutant non-small cell lung cancer progression

Fig. 3

Overexpression of miR-199b dramatically inhibits K-Ras mutation-driven lung tumorigenesis and progression. a-c Overexpression of miR-199b inhibited cell proliferation, soft agar colony formation and cell invasion in both K-Ras-mutated NSCLC cell lines, A549 and H2122. Cells were transfected with negative control oligonucleotides (NC) or miR-199b mimics and then subjected to cell proliferation, soft agar colony formation and invasion assays. d-e Overexpression of miR-199b inhibited tumor growth and cancer cell proliferation in the A549 xenograft models. Stably expressing miR-199b A549 and vector control cells were used to generate a xenograft model in nude mice. Tumors were collected 1 month after cell injection, and the tumor weights were measured. Cell proliferation was analyzed using Ki-67 IHC in tumor tissues. f Overexpression of miR-199b inhibited NSCLC cell metastasis in vivo. Stably expressing miR-199b A549 or control cells were injected into nude mice by tail vein injection. The mice were sacrificed 1 month after cell injection, and the tumors on the lung surface were counted. g Aerosol delivery of miR-199b to the lungs inhibits K-Ras mutation-driven lung tumorigenesis. K-RasLA1 transgenic mice were exposed to a mixture of miR-199b expression plasmid with the gene carrier (miR-199), gene carrier only (Carrier) or empty plasmid only (vector) using a nose-only aerosol delivery system. Four weeks after gene delivery, mice were sacrificed, and the number of tumor nodules on the lung surface were counted. h Aerosol delivery of miR-199b inhibited cancer cell proliferation in lung tumor of K-RasLA1 transgenic mice. The Ki-67 expression was detected using immunohistochemistry

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