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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Fn14 overcomes cisplatin resistance of high-grade serous ovarian cancer by promoting Mdm2-mediated p53-R248Q ubiquitination and degradation

Fig. 2

The effects of Fn14 on cisplatin-resistance in HGSOC cells. (a) CCK-8 assay was performed to detect cell viability in OVCAR-3 cells,*p < 0.05. (b) Western blot analysis detecting the DNA damage maker in OVCAR-3 cells infected with Fn14 lentivirus or the control lentivirus. (c) CCK-8 assay was performed to detect cell viability in SKOV-3 cells. (d) Western blot analysis detecting the DNA damage maker in SKOV-3 cells transfected with the Fn14 siRNA or negative control. (e) and (f) Western blot analysis detecting the activation of Caspase-3 and the expression of Bcl-2 in OVCAR-3 and SKOV-3 cells after diffident treatment. (g) and (h) Effects of Fn14 on apoptosis of OVCAR-3 cells were determined by flow cytometry analysis, **p < 0.01. (i) Genetic Alteration of p53 in HGSOC. Unpaired Student’s t test was performed for comparisons between two groups

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