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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Fn14 overcomes cisplatin resistance of high-grade serous ovarian cancer by promoting Mdm2-mediated p53-R248Q ubiquitination and degradation

Fig. 4

Fn14 inhibits cisplatin resistance in HGSOC primary cancer cells with p53-R248Q. (a) CCK-8 assay was performed to detect cell viability in HGSOC cells transfected with p53 siRNA or negative control. (b) Western blot showing the levels of p53 R248Q in HGSOC cells transfected with p53 siRNA. (c) Western blot analysis detecting the the expression of p53-R248Q in HGSOC cells after infection with Fn14 lentivirus. (d) and (e) qRT-PCR detecting the expression of p53-R248Q target genes in HGSOC cells after infection with Fn14 lentivirus, *p < 0.05, **p < 0.01, ***p < 0.001. (f-h) CCK-8 assay was performed to detect IC50 of CDDP in HGSOC cells co-infected with Fn14 and p53 R248Q lentivirus, *p < 0.05, **p < 0.01. Unpaired Student’s t test was performed for comparisons between two groups

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