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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: FOXO1-regulated lncRNA LINC01197 inhibits pancreatic adenocarcinoma cell proliferation by restraining Wnt/β-catenin signaling

Fig. 2

LINC01197 is a direct target of FOXO1 and is mainly localized in the cell nucleus. a LINC01197 expression is significantly decreased in PDAC cells compared to that in HPNE cells. b Altering the FOXO1 expression level similarly changed LINC01197 expression in AsPC1, BxPC3, PANC1, and HPNE cell lines. c Schematic representation of binding site for FOXO1 transcription factor in promoter region of the Linc01197 gene. Luciferase assay was carried out in PANC1 cells co-transfected with PGL3-3DBE or Linc01197 promoter and FOXO1 at different concentration for 48 h. d Northern blot assay showing LINC01197 expression level in HPNE and PANC1 cells. e Nucleocytoplasmic separation and FISH assays showed that LINC01197 is mainly localized in HPNE cells. P < 0.05 indicates a significant difference and all assays were performed in triplicate

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