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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: FOXO1-regulated lncRNA LINC01197 inhibits pancreatic adenocarcinoma cell proliferation by restraining Wnt/β-catenin signaling

Fig. 4

LINC01197 directly binds to β-catenin and remarkably inhibits the Wnt/β-catenin signaling pathway in PDAC cell lines. a and b Western blot of input and β-catenin complexes pulled down by LINC01197, antisense control from nuclear extracts of PANC1. c RIP assays showed that LINC01197 was enriched by β-catenin in indicated cell lines. d Dual luciferase reporter assays revealed that overexpression of LINC01197 inhibited Wnt/β-catenin signaling activity (TCF/β-catenin reporter) in PANC1 and BxPC3. e qRT-PCR showed MYC, CCND1, CD44, and MMP26 expression levels were significantly decreased when PANC1 and BxPC3 cells overexpressed LINC01197. P < 0.05 indicates a significant difference and all assays were performed in triplicate

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