Fig. 2

Effect of pioglitazone treatment on colony formation in H1299 and H460 lung cancer cells and Beas2B normal cell line. Cells were treated with pioglitazone (range 0.1–10 μM) for 72 h. Colonies were stained with 0.1% crystal violet and counted as described in Materials and Methods. a Histogram of colony number counted by imageJ plugin. Asterisks indicate statistical significance, P < 0.05. b Representative images of lung cancer cells (H460) acquired by phase-contrast microscope