Fig. 7

Effect of pioglitazone on cellular metabolism. Analysis of glycolysis by SeaHorse in lung cancer cells following pioglitazone treatment (range 0.1–10 μM) for 30 min and 2 h. a, b Graphical representation of extracellular acidification rate (ECAR) in H1299 and H460 cell lines: each colored curve indicated concentration of pioglitazone treatment: for H1299 (a): green, untreated control; yellow, 0.1 μM; light blue, 1 μM, and purple, 10 μM. For H460 (b): yellow, untreated control; red,0.1 μM; light blue,1 μM, dark blue, 10 μM. c, d Histograms represented the ECAR quantification time dependent, in H1299 (c) and H460 (d) cell lines. P values < 0.05 were considered as statistically significant (**). e Expression of Glut-1, TKLT1, SLC15A and G6PD evaluated by immunoblotting in H1299 and H460 cell lines. +: pioglitazone 10 μM. α-tubulin was used as the loading control