Fig. 2

SNHG1 activates of the Akt pathway by regulating SLC3A2 in HCC cells. a and b HepG2, HepG2-SR, Huh7 and Huh7-SR cells were incubated with sorafenib (2.5 μM) for 48 h, and then subjected to qRT-PCR for detecting SLC3A2 mRNA (a) and Western blot analysis for detecting SLC3A2 protein (b). c and d Huh7 and Huh7-SR cells were untreated (mock) or transfected with SNHG1 Smart Silencer (anti-SNHG1) for 48 h. Cells were subjected to RT-PCT for detecting mRNA expressions (c) and Western blot analysis for detecting protein expressions (d). The mRNA expression level in untreated Huh7 cells was defined as 1. The density of Western blot band was normalized to β-actin. N.S., no significance. “*” (P < 0.05) and “**” (P < 0.001) indicate a significant difference