Fig. 3

Depletion of SNHG1 enhances the effects of sorafenib in promoting apoptosis and autophagy of sorafenib-resistant cells. Huh7-SR cells either untreated (control) or transfected with SNHG1 Smart Silencer (anti-SNHG1) were incubated for 48 h in the absence or presence of sorafenib (5 μM). a Representative dot plots were from the above cytometrically analyzed cells. b Representative images were taken from the above cells stained by Annexin V/propidium iodide or acridine orange (magnification × 200). c Cell apoptosis (%) was measured by cytometry. d The fold change of acridine orange fluorescence intensity (FL3) versus the untreated controls, which was defined as 1, was calculated. e Cells were subjected to Western blot analysis. The density of each band was normalized to β-actin. “*” (P < 0.05) and “**” (P < 0.001) indicate a significant difference