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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: MiR-29b/Sp1/FUT4 axis modulates the malignancy of leukemia stem cells by regulating fucosylation via Wnt/β-catenin pathway in acute myeloid leukemia

Fig. 2

FUT4 mediates the progression of CD34 + CD38-AML cell lines in vitro and in vivo. a The FUT4 mRNA (left panel) and protein (right panel) levels were detected by qRT-PCR and western blot in transfected LSCs-KG-1a/MOLM13 cells. b Altered LTL level of transfected LSCs cells was shown by flow cytometry. c Lower cell viability was measured by CCK8 assays in LSCs cells transfected with shFUT4. d Sphere formation ability was attenuated by shFUT4 transfection. e Immunoflurescence of Ki67 was decreased after knocking down FUT4. f The apoptotic rates of transfected LSCs-KG-1a cells were measured by flow cytometry. g The occurrence of apoptosis was determined by TUNEL assay. h Cleaved caspase3 and PARP levels were upregulated in shFUT4-LSCs by western blot. i Drug resistance to ADR, Ara-C and Pacilitaxel was determined by CCK8 assays. j Intensity of P-gp was weakening with down-regulating FUT4 in KG-1a cells by flow cytometry. k The tumorigenesis of transfected LSCs-KG-1a cells in vivo was presented with or without ADR treatment. (l) FUT4 and Ki67 levels were determined by immunohistochemistry staining. Data are the means ± SD of triplicate determinants (*P < 0.05)

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