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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: MiR-29b/Sp1/FUT4 axis modulates the malignancy of leukemia stem cells by regulating fucosylation via Wnt/β-catenin pathway in acute myeloid leukemia

Fig. 3

Sp1 mediates FUT4 transcription and translation in CD34 + CD38- AML cell lines. a Sp1 protein level was determined by western blot between LSCs and non-LSCs of KG-1a cells. b Sp1 protein level was determined by western blot between LSCs and non-LSCs of MOLM13 cells. c The promoters of FUT4 were presented, and the predicted transcription factor binding elements (TBE) were indicated. The fragments of FUT4 promoter were investigated. d Directly binding between Sp1 and FUT4 promoter was verified by dual-luciferase reporter assays. e The P3 fragment of FUT4 promoter showed directly interaction with Sp1 by dual-luciferase reporter assays. f The binding of FUT4 promoter and Sp1 was further explored by ChIP-PCR assays. g With the mediation of Sp1, the binding affinity between Sp1 and FUT4 promoter was detected in AML cell lines. Data are the means ± SD of triplicate determinants (*P < 0.05)

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