Fig. 1

PTEN is poorly expressed in glioma and predicted to be a target of miR-26a. a GO enrichment analysis of DEGs in expression dataset of glioma (GSE50161); three histograms refer to the biological process (BP), cellular component (CC) and molecular function (MF) enrichment, respectively; the abscissa refers to GO entry, while the ordinate refers to the number of DEGs; b the intersection of DEGs and the known genes related to glioma. Two circles represent DEGs obtained from GSE50161 datasets (blue) and the known genes related to glioma obtained from DisGeNET (red), and the middle part indicates their intersection; c interaction analysis of glioma DEGs; each circle represents one gene, the line among circles represents the interaction among genes, and the color of circle represents the core degree of gene in the whole network; d PTEN expression in GSE50161 dataset; the abscissa refers to sample type, and the ordinate refers to gene expression level; the left box plot refers to the normal group, and the right box plot refers to the glioma group; e putative miRNAs that regulate PTEN; the intersection of putative miRNAs targeting PTEN obtained from the four databases and up-regulated miRNAs in glioma from GSE90604 dataset; the middle part refers to the intersections among five datasets; f the expression of miR-92b-3p, miR-19a-3p, miR-19b-3p, hsa-miR-4429 and miR-26a in control brain tissues and glioma tissues determined by RT-qPCR; the data between control brain tissues (n = 28) and glioma tissues (n = 46) are compared by unpaired-t test. The experiments are repeated 3 times. * p < 0.05, compared with the control group. DEGs, differentially expressed genes; miR/miRNA, microRNA; GO, gene oncology; PTEN, phosphatase and tensin homolog deleted on chromosome ten