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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Glioma stem cells-derived exosomal miR-26a promotes angiogenesis of microvessel endothelial cells in glioma

Fig. 3

GSCs deliver miR-26a to HBMECs through exosomes. a miR-26a expression in GSCs transfected with miR-26a agomir and their derived exosomes using RT-qPCR; b miR-26a expression and PTEN expression in HBMECs co-cultured with exosomes derived from miR-26a agomir-transfected GSCs using RT-qPCR; c exosomes and uptake of exosomes into HBMECs observed under the confocal microscope (× 400); exosomes labeled with PKH26 was stained red; GFP-labeled HBMECs were stained green; the nucleus stained with DAPI was blue; *, p < 0.05, compared with the GSCagomir-NC or control group; all data were expressed as mean ± standard deviation; comparisons between two groups were analyzed using unpaired t-test; comparisons among multiple groups were analyzed by the one-way ANOVA; the experiment was repeated three times; ANOVA, analysis of variance; RT-qPCR, reverse transcription quantitative polymerase chain reaction; miR-26a, microRNA-26a; GFP, green fluorescent protein; PTEN, phosphatase and tensin homolog deleted on chromosome ten; HBMECs, microvascular endothelial cells; GSCs, glioma stem cells; DAPI, 4′,6-diamidino-2-phenylindole; NC, negative control

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