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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Glioma stem cells-derived exosomal miR-26a promotes angiogenesis of microvessel endothelial cells in glioma

Fig. 6

Exosomal miR-26a promotes proliferation and angiogenesis of HBMECs through the inhibition of PTEN and activation of PI3K/Akt signaling pathway. a VEGF level in HBMECs co-cultured with GSC-EXsagomir-NC or GSC-EXsmiR-26a agomir; b PTEN expression in HBMECs co-cultured with GSC-EXsagomir-NC or GSC-EXsmiR-26a agomir; c Akt protein level and phosphorylation level in HBMECs co-cultured with GSC-EXsmiR-26a + PTEN or GSC-EXsmiR-26a + PTEN-NC; d proliferation of HBMECs co-cultured with GSC-EXsagomir-NC or GSC-EXsmiR-26a agomir; e migration of HBMECs co-cultured with GSC-EXsagomir-NC or GSC-EXsmiR-26a agomir; f tube formation in HBMECs co-cultured with GSC-EXsagomir-NC or GSC-EXsmiR-26a agomir; g VEGF protein level in HBMECs co-cultured with GSC-EXsagomir-NC or GSC-EXsmiR-26a agomir; *, p < 0.05, compared with the control or GSC-EXsagomir-NC group; all data were expressed as mean ± standard deviation; comparisons between two groups were analyzed using unpaired t-test; comparisons among multiple groups were analyzed by the one-way ANOVA; the experiment was repeated three times; ANOVA, analysis of variance; miR-26a, microRNA-26a; NC, negative control; PTEN, phosphatase and tensin homolog deleted on chromosome ten; Akt, protein kinase B; HBMECs, human brain microvascular endothelial cells; VEGF, vascular endothelial growth factor; GSC-EXsagomir-NC, exosomes derived from agomir-NC-transfected GSCs; GSC-EXsmiR-26a agomir, exosomes derived from miR-26a agomir-transfected GSCs

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