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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Glucocorticoid modulatory element-binding protein 1 (GMEB1) interacts with the de-ubiquitinase USP40 to stabilize CFLARL and inhibit apoptosis in human non-small cell lung cancer cells

Fig. 3

GMEB1 physically interacted with CFLARL in NSCLC cells. a, b Co-IP assays were conducted in HEK293FT cells using FLAG-CFLARL and HA-GMEB1 plasmids. c GST-pull down assay was conducted in A549 cells using GST-CFLARL plasmids. d IP assays was conducted in A549 and H1299 cells using anti-FLIPL (Santa Cruz, US) antibody. e Calu-1 cells were fixed and subjected to indirect immunofluorescence staining with anti-FLIPL (Santa Cruz, US) and GMEB1 (Santa Cruz, US). The red signal (CFLARL) was obtained with anti-rabbit IgG Alexa 568-conjugated secondary Ab, and the green signals (GMEB1) were obtained with anti-mouse IgG Alexa 488-conjugated secondary Ab. Nuclei were stained with DAPI

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