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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Nalbuphine suppresses breast cancer stem-like properties and epithelial-mesenchymal transition via the AKT-NFκB signaling pathway

Fig. 5

Nalbuphine inhibits breast cancer stem-like properties and EMT through the AKT-NFκB pathway. a MDA-MB-231 cells were treated with the indicated concentration of nalbuphine for 48 h and levels of the indicated proteins were determined by western blot (n = 3). b MDA-MB-231 cells were treated with nalbuphine and/or SC79 for 48 h and levels of indicated proteins were determined by western blot (n = 3). c Representative spheroid images derived from MDA-MB-231 cells treated with nalbuphine and/or SC79 (upper) (n = 3); Scale bar, 50 μm. Mammosphere sizes (bottom left) and number of mammospheres (bottom right) (d > 50 μm) are shown. d Representative tumor image (left) and growth curves (right) of mice with indicated treatment (n = 5). e MDA-MB-231 cells were treated with nalbuphine and/or SC79 for 48 h and then subjected to wound healing analysis. Representative images (left) and statistical analysis (right) are shown (n = 3). Data are presented as mean ± SEM. p-value was determined by Student’s t-test and ANOVA (c and d) (*p < 0.05, **p < 0.01, ***p < 0.001). See also Additional file 6: Figure S5

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