Fig. 3

MTMR2 induces epithelial-mesenchymal transition in GC cells. a, b Representative images of morphology showed that silencing MTMR2 resulted in reduced cellular protrusions (a), while overexpressing MTMR2 resulted in not only increased cellular protrusions but also more spindle-like shape in partial cells in MGC803 and BGC823 GC cells (b). c qRT-PCR analyses showed that MTMR2 knockdown upregulated E-cadherin and downregulated N-cadherin and vimentin at mRNA level in MGC803 and BGC823 cells, ^*, P < 0.05; ^**, P < 0.001. d qRT-PCR analyses showed that MTMR2 overexpression downregulated E-cadherin and upregulated N-cadherin and vimentin at mRNA level in MGC803 cells and BGC823 cells, *, P < 0.05; **, P < 0.01. e Western blot analyses showed that MTMR2 knockdown upregulated E-cadherin and downregulated N-cadherin and vimentin at protein level in MGC803 and BGC823 cells. f Western blot analyses showed that MTMR2 overexpressing downregulated E-cadherin and upregulated N-cadherin and vimentin in MGC803 and BGC823 cells. g MTMR2 expression was correlated with the expression of E-cadherin, N-cadherin and vimentin in GC samples from TCGA dataset (P < 0.001 for all)