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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: MTMR2 promotes invasion and metastasis of gastric cancer via inactivating IFNγ/STAT1 signaling

Fig. 6

IRF1 mediates the inhibition of IFNγ/STAT1 on ZEB1 in GC cells. a, b Silencing of MTMR2 upregulated the expression of STAT1 targeted genes at both mRNA (a) and protein (b) levels, including IRF9, IRF1, IFITM1 and TAP1, with the most significant upregulation of IRF1 in MGC803 and BGC823 cells. c Silencing IRF1 significantly increased the invasion capacity in mock cells, and abrogated the inhibitory effect of MTMR2-knockdown on the invasion in sh-MTMR2 MGC803 and BGC823 cells. d Silencing IRF1 with siRNA (40 nmol/L) downregulated E-cadherin and upregulated N-cadherin and vimentin in mock MGC803 and BGC823 cells, and attenuated MTMR2 knockdown-induced upregulation of E-cadherin and downregulation of N-cadherin and vimentin in sh-MTMR2 MGC803 and BGC823 cells. e IRF1 siRNA (40 nmol/L) significantly increased ZEB1 promoter activity in mock MGC803 and BGC823 cells and sh-MTMR2 MGC803 and BGC823 cells, **, P < 0.01, ***, P < 0.001. f ChIP assay showed a candidate binding region (4F to 4R, − 1 to − 165) of IRF1 in ZEB1 promoter. g Representative images of IHC staining of pSTAT1, IRF1 and ZEB1 in intraperitoneal metastasis tumor tissue sections (original magnification, × 400) and the IHC scores of pSTAT1, IRF1 and ZEB1 protein expression, * P < 0.05

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