Fig. 3

Endogeneous concentration of EETs and protein expression of related CYP epoxygenases correlates with receptor status of human-derived breast cancer cell lines. a Box plots show the intracellular concentration of AA-derived EETs and b corresponding EET/DHET metabolite ratios in human-derived TNBC (MBA-MB-231, Hs 578 T, MDA-MB-468, HCC1937 and BT-20), ER+/PR+/HER2– (MCF7, ZR75-1 and BT483), HER2-overexpressing (SKBR3, MDA-MB-361 and BT474) cell lines and normal mammary epithelial cells (MCF10A) analyzed using UPLC-MS/MS spectrometry. All analyses include 3 biological replicates and 4 technical replicates. Error bars indicate mean ± SEM. Significantly different values (P = 0.05, ANOVA) are denoted by letters. c Western blotting results show protein expression levels of CYP epoxygenases, CYP2J2 and CYP2C9/19, and sEH, in human mammary-derived cancer or normal cell lines. Representative blots of three independent experiments are shown