Fig. 5

Exogenous EETs, alone or in combination, promote migration, invasion and metastasis phenotype in mesenchymal-like TNBC cells. a Cell viability, b motility, c migration, invasion, and colony formation following treatment with the four EET isomers in combination (cell viability: 1–40 nM each, total EET concentration of 4–160 nM; other assays: 2.5 nM each isomer, total EET concentration of 10 nM) for 24 h for representative cell lines of each breast cancer subtype were measured. d Increased cell migration and invasion was observed following 24 h 10 nM EET treatment, with or without 14,15-EEZE in mesenchymal TNBC cells but not in the other breast cancer cell subtypes. Error bars indicate mean ± SEM. Significantly different values (P = 0.05, ANOVA) are denoted by letters. All analyses include 3 biological replicates and 4 technical replicates. Error bars indicate mean ± SEM. Significantly different values (P = 0.05, ANOVA) are denoted by letters. Capitalized letters are for the migration data comparison and analysis. e Activation and expression levels of metastasis-related proteins in MDA-MB-231 cells were observed following 6 and 24 h treatment with 10 nM EETs. Representative blots of three independent experiments are shown