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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Long non-coding RNA AGAP2-AS1, functioning as a competitive endogenous RNA, upregulates ANXA11 expression by sponging miR-16-5p and promotes proliferation and metastasis in hepatocellular carcinoma

Fig. 3

AGAP2-AS1 promotes tumor growth and metastasis in vivo. a Representative HE staining of lung metastases in AGAP2-AS1 overexpression or knockdown cells. b Immunohistochemistry of E-cadherin and vimentin were showed and compared between tissues of respective AGAP2-AS1 expression level. c Tumor growth curve revealed that AGAP2-AS1 overexpression significantly promoted, while AGAP2-AS1 knockdown inhibited tumor growth in vivo. Tumor nodules were subjected to immunohistochemical staining for Ki-67 (d) and TUNEL (e) assays and quantitative analysis. Representative immunostaining and TUNEL assays revealed that AGAP2-AS1 overexpression significantly increased the number of Ki-67 positive cells and inhibited the number of apoptotic cells. However, the percentage of Ki-67 positive cells in tumors arising from the AGAP2-AS1 knockdown group was significantly lower and the percentage of apoptotic cells was significantly higher than that in the negative control group. e Immunohistochemistry of E-cadherin and Vimentin were showed and compared between AGAP2-AS1 high expressing HCC tissues and AGAP2-AS1 low expressing cases. *P < 0.05

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