Fig. 2

miR-let-7b and miR-let-7c inhibited growth of mucosal melanoma cells in vitro and in vivo. The expression of miR-let-7b (a) and miR-let-7c (b) was downregulated in mucosal melanoma cell lines. The expression was detected through qPCR with the internal control RNU6B in melanocytes. The results are presented as the means ± standard deviations from triplicate experiments and statistically analysed by Mann-Whitney U test. miR-let-7b and miR-let-7c inhibited the growth of HMVII (c) and GAK (d) in vitro. The cell viability was determined using the Cell Titer-Glo luminescent cell viability assay. e miR-let-7b and miR-let-7c inhibited the growth of HMVII in vivo. f Representative images of xenografts models stratified by miR-let-7b and miR-let-7c expressions. Lentiviral infection HMVII cells were subcutaneously inoculated into NOD/SCID mice. Each group contained 5 mice. The results are presented as the means ± standard deviations and statistically analysed by repeated measures analysis of variance. *P < 0.05