Fig. 5

miR-let-7b and miR-let-7c inhibited MTDH and CALU via binding to their 3′ UTRs. The sequences of miR-let-7b, miR-let-7c and potential binding sites in the 3′ UTRs of target genes MTDH (a) and CALU (b) were predicted by software packages. miR-let-7b and miR-let-7c inhibited the luciferase activity of wild-type MTDH (c) and CALU (d) 3′ UTR in 293 T cell lines. The results are presented as the means ± standard deviations from six replicates and statistically analysed by Mann-Whitney U test. e Western blotting was used to detect proteins influenced by miR-let-7b and miR-let-7c; GAPDH is shown as a loading control. The relative intensity of MTDH (f), CALU (g) and phospho-ERK (h) influenced by miR-let-7b and miR-let-7c were quantified by Image J software and analysed statistically. The relative intensity of MTDH and CALU were normalized to GAPDH, and phosphorylated bands were normalized to total bands. The results are presented as the means ± standard deviations from triplicate scans and statistically analysed by Mann-Whitney U test. *P < 0.05