Fig. 6

MTDH and CALU partially reversed the function of miR-let-7b and miR-let-7c in vitro. a Western blotting was used to detect MTDH and CALU influenced by co-transfection of miR-let-7b/miR-let-7c and MTDH/CALU in HMVII and GAK; GAPDH is shown as a loading control. b The relative intensity of MTDH and CALU after co-transfection was quantified by Image J software and analysed statistically. The relative intensity of MTDH and CALU were normalized to GAPDH. The results are presented as the means ± standard deviations from triplicate scans and statistically analysed by Mann-Whitney U test. MTDH and CALU reversed cell growth (c) and apoptosis (d and e) in response to miR-let-7b and miR-let-7c in HMVII and GAK cells. The cell viability was determined using the Cell Titer-Glo luminescent cell viability assay. The results are presented as the means ± standard deviations and statistically analysed by repeated measures analysis of variance. The cell apoptosis (e) results are presented as the means ± standard deviations of triplicate experiments and statistically analysed by Mann-Whitney U test. *P < 0.05