Fig. 5

Effect of PDK1 downregulation on signalling pathways in PDAC cell lines. AsPC-1 (a), CFPAC-1 (b) and HPAF-II (c) were transfected with the indicated siRNAs and lysed 72 h post-transfection. Lysates were then analysed by Western blotting using the indicated antibodies. Representative blots are shown. In all blots GAPDH was used as loading control. *indicates membranes that derived from the same gel (therefore only one GAPDH is shown)