Fig. 6

siRNA-mediated SGK3 downregulation inhibits PDAC cells growth. AsPC-1 (a), CFPAC-1 (b) and HPAF-II (c) cells were transfected with two siRNAs specifically targeting SGK3 or a non-targeting siRNA (siControl) and then manually counted with trypan blue exclusion as specified in the Methods. Data are expressed as percentage of cells transfected with siControl and are means ± SEM of n ≥ 3 independent experiments performed in duplicate. T-test and GraphPad Prism version 6.0 were used for statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001. (d) Representative blot confirming efficient SGK3 downregulation in all cell lines. GAPDH was used as loading control