Fig. 1

Long non-coding RNA TPTEP1 sensitizes cisplatinum-induced apoptosis in HCC cells. a The heatmap was drawn to show the differentially expressed LncRNAs in hepatocellular carcinoma QGY-7703 cells-treated with cisplatinum (30μg/ml) for the indicated time points by RNA sequencing. b Relative expression levels of the ten indicated LncRNAs in cisplatinum-treated QGY-7703 cells were analyzed by qRT-PCR. c QGY-7703 cells were transfected with the indicated siRNAs for 24 h and then treated with cisplatinum (30μg/ml) for 24 h. The cell apoptotic rates were analyzed by flow cytometry. d The relative expression levels of TPTEP1 in the cytoplasm and nucleus of QGY-7703 cells treated with cisplatinum (30μg/ml) or not for 12 h were analyzed by qRT-PCR (TfIIb as the cytoplasmic maker, and NEAT1 as the nuclear maker). e The relative expression levels of TPTEP1 in the indicated HCC cell lines and L02 human normal liver cells were analyzed by qRT-PCR. TPTEP1 expression in L02 cells was used for the control. Data are represented as means ± SD (n = 3; ^*represents P < 0.05)