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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Long non-coding RNA TPTEP1 inhibits hepatocellular carcinoma progression by suppressing STAT3 phosphorylation

Fig. 3

Long non-coding RNA TPTEP1 directly interacts with STAT3 to suppress HCC cell progression. a Biotin labeled TPTEP1 was generated through in vitro transcription, and incubated with QGY-7703 lysate for RNA pulldown, and the denoted band of TPTEP1 (Sense) was identified as STAT3 by Mass spectrum. b The interactions between TPTEP1 and STAT3 were detected by RIP assays in QGY-7703 and MHCC97H cells. c Cell apoptotic rates were analyzed by flow cytometry in TPTEP1-overexpressed QGY-7703 cells (LV-TPTEP1) with or without STAT3 overexpression (pCMV-STAT3) under cisplatinum treatment. d Cell prolifiration was examined by MTT assays in TPTEP1-overexpressed QGY-7703 cells (LV-TPTEP1) with or without STAT3 overexpression (pCMV-STAT3) e Cell invasive ability was examined by transwell invasion assays in TPTEP1-overexpressed QGY-7703 cells (LV-TPTEP1) with or without STAT3 overexpression (pCMV-STAT3). Data are represented as means ± SD (n = 3; *represents P < 0.05)

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