Fig. 3From: PKM2 is the target of proanthocyanidin B2 during the inhibition of hepatocellular carcinomaPKM2 is the target of PB2, and the overexpression or knockdown of PKM2 influenced the effect of PB2 in HCC cell lines. a The fluorescence of HCC cells after transfection with PKM2-OE or sh-PKM2 lentiviruses (original magnification, 200×). b, C The transfection efficiency was verified by qPCR and western blotting. *P < 0.05 vs. the NC group. d The effects of PKM2 overexpression or knockdown on proliferation- and apoptosis-related markers, by western blotting. e The effects of PB2 on the proliferation and apoptosis in PKM2-OE or sh-PKM2 HCC cells. f The effects of PB2 on the glucose uptake and lactate levels in PKM2-OE or sh-PKM2 HCC cells. g The gross images of tumors in EV, PKM2-OE, or PKM2-OE + PB2 groups (bars: 10 mm). h The tumor volumes and tumor tissue lactate levels in EV, PKM2-OE, or PKM2-OE + PB2 groups. i The H&E staining and IHC staining of PKM2, GLUT1 (bars: 100 μm). *P < 0.05 vs. the PKM2-OE group; # P < 0.05 vs. the EV group (3b: one-way ANOVA; 3f & 3h: Student’s t-test)Back to article page