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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: PKM2 is the target of proanthocyanidin B2 during the inhibition of hepatocellular carcinoma

Fig. 3

PKM2 is the target of PB2, and the overexpression or knockdown of PKM2 influenced the effect of PB2 in HCC cell lines. a The fluorescence of HCC cells after transfection with PKM2-OE or sh-PKM2 lentiviruses (original magnification, 200×). b, C The transfection efficiency was verified by qPCR and western blotting. *P < 0.05 vs. the NC group. d The effects of PKM2 overexpression or knockdown on proliferation- and apoptosis-related markers, by western blotting. e The effects of PB2 on the proliferation and apoptosis in PKM2-OE or sh-PKM2 HCC cells. f The effects of PB2 on the glucose uptake and lactate levels in PKM2-OE or sh-PKM2 HCC cells. g The gross images of tumors in EV, PKM2-OE, or PKM2-OE + PB2 groups (bars: 10 mm). h The tumor volumes and tumor tissue lactate levels in EV, PKM2-OE, or PKM2-OE + PB2 groups. i The H&E staining and IHC staining of PKM2, GLUT1 (bars: 100 μm). *P < 0.05 vs. the PKM2-OE group; # P < 0.05 vs. the EV group (3b: one-way ANOVA; 3f & 3h: Student’s t-test)

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