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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: The TGFβ-miR-499a-SHKBP1 pathway induces resistance to EGFR inhibitors in osteosarcoma cancer stem cell-like cells

Fig. 5

miR-499a directly targets SHKBP1 expression and SHKBP1 was negatively correlated with miR-499a level in OS tissues. a Illustration of SHKBP1 3’UTR as well as the seed sequence of miR-499a showed the predicted target region on the 3’UTR of SHKBP1 mRNA. b Dual-luciferase reporter assay with co-transfection SHKBP1 3’UTR plasmids and miR-499a mimics. The relative luciferase activity was obtained by firefly luciferase activity normalized against Renilla luciferase activity. Note: Columns, mean of three individual experiments; SD,**, P < 0.01. c Down-regulation of miR-499a was observed in OS tissues compared with that in adjacent ones by qPCR (left). Up-regulation of SHKBP1 was observed in OS tissues compared with that in adjacent ones by qPCR (right). Note: Columns, mean of three individual experiments; SD,**, P < 0.01

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