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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Anti-EMT properties of CoQ0 attributed to PI3K/AKT/NFKB/MMP-9 signaling pathway through ROS-mediated apoptosis

Fig. 4

CoQ0 inhibits EMT through up-regulation of E-cadherin signaling pathways in MDA-MB-231 cells. a-d Cells were treated with CoQ0 (0.5–2 μM) for 24 h. a Morphological changes were examined by phase-contrast microscope (200× magnification). b Transcriptional activity of E-cadherin was monitored by luciferase reporter assay. c Immunofluorescence analysis for E-cadherin protein expression. d CoQ0-induced modulation of epithelial (E-cadherin and Occludin) and mesenchymal marker proteins (Vimentin, Slug, Twist, and Snail) were monitored using Western blot analyses. e mRNA expression of E-cadherin, Vimentin, Slug, and Snail after 6 h treatment with CoQ0 (0.5–2 μM) was measured by RT-PCR analyses. As internal control GAPDH was used. The results are presented as the mean ± SD of three independent assays. **p < 0.05, ***p < 0.001 significant compared to control cells

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