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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Anti-EMT properties of CoQ0 attributed to PI3K/AKT/NFKB/MMP-9 signaling pathway through ROS-mediated apoptosis

Fig. 6

CoQ0 inhibits TGF-β/TNF-α-induced metastasis and EMT in MCF-10A cells. Cells were pretreated with 2 μM CoQ0 for 1 h and then stimulated with TGF-β/TNF-α (10 ng/mL) for 24 h. (A-C) CoQ0 inhibits TNF-α/TGF-β-induced metastasis. a Cells invasiveness determined by counting cells in three microscopic fields per sample. b CoQ0 inhibits TNF-α/TGF-β-induced MMP-2/− 9 and uPA. Inhibition of MMP-2 and MMP-9 activity in conditioned medium from MCF-10A cells was evaluated using gelatin zymography. c CoQ0 inhibits TNF-α/TGF-β-induced uPA. uPA protein expression was monitored by using Western blot analyses. (D-E) CoQ0 inhibits TNF-α/TGF-β-induced EMT. d Cytoskeletal pattern of F-actin was measured by immunofluorescence analyses (100 × magnification). e CoQ0-induced TNF-α/TGF-β decreased E-cadherin and inhibited TNF-α/TGF-β-induced β-catenin. Using Western blot analyses monitored e-cadherin and β-catenin protein expression. The results are presented as the mean ± SD of three independent assays. **p < 0.05, ***p < 0.001 significant compared to control cells; ##p < 0.01, ###p < 0.001 significant compared to TNF-α alone treated cells

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