Fig. 7
CoQ0 induces apoptosis through ROS generation in MDA-MB-231 cells. a-b CoQ0 induced ROS generation. Cells were treated with CoQ0 (15 μM) for 0–60 min. The intracellular ROS level as a percentage of the control, is expressed in graphical data. c-e Cells were pre-treated with antioxidant N-acetylcysteine (NAC, 1 mM) for 1 h followed by treatment with CoQ0 (5–15 μM for 15 min) and were analyzed for ROS generation (c and d) and cell viability (e). f Cells were pre-treated with NAC (1 mM) for 1 h followed by CoQ0 (7.5 μM) treatment for 24 h. The expression of Bax and p53 was monitored by Western blot. The results are presented as the mean ± SD of three independent assays. **p < 0.05, ***p < 0.001 significant compared to control cells