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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Inhibition of the aberrant A1CF-FAM224A-miR-590-3p-ZNF143 positive feedback loop attenuated malignant biological behaviors of glioma cells

Fig. 5

ZNF143 was a target of miR-590-3p and could rescue the tumor-suppressive impacts of miR-590-3p. a. The ZNF143 expression of glioma cells after A1CF and FAM224A knockdown was showed. b. The ZNF143 expression of glioma cells treated with altering miR-590-3p expression was illustrated. c. The ZNF143 expression of glioma cells co-transfected with sh-FAM224A and anti-miR-590-3p was exhibited. d. The potential miR-590-3p binding sites in ZNF143 3′ UTR and the designed mutant sequence were indicated. e. Relative luciferase activity was evaluated after cells were co-transfected with pre-miR-590-3p and ZNF143–3′ UTR-Wt or ZNF143–3′ UTR-Mut. Data were presented as the mean ± SD (n = 5, each group). **P < 0.01 vs. ZNF143–3′ UTR Wt + pre-NC group. f-h. CCK-8 assay, flow cytometry analysis and migration and invasion assays were conducted to determine the biological behaviors of glioma cells co-transfected with miR-590-3p and ZNF143. Data represented mean ± SD (n = 5, each group). **P < 0.01 vs. pre-NC + ZNF143 (+)-NC group, ##P < 0.01 vs. pre-miR-590-3p + ZNF143 (+)-NC group. Scale bar of migration and invasion assays assay represent 40 μm

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