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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: ROS-mediated activation and mitochondrial translocation of CaMKII contributes to Drp1-dependent mitochondrial fission and apoptosis in triple-negative breast cancer cells by isorhamnetin and chloroquine

Fig. 4

Genetic interruption of CaMKII blocks mitochondrial fission and apoptosis induced by CQ/IH combination. MDA-MB-231 cells transfected with either wild type CaMKII (WT) or mutants CaMKIIT286A (T286A) or CaMKIIT286D (T286D) were treated with CQ (20 μM) in the presence or absence of IH (10 μM) for 48 h. a The expressions of p-CaMKII in WCL, and CaMKII and Drp1 in Mito were examined by Western blot. b The colocalization of CaMKII (red), Drp1 (green), and MitoTracker (blue) was examined by confocal microscopy. Scale bars: 10 μm. c Apoptosis was detected by flow cytometry analysis. The values represent the mean ± SD for three separate experiments (**P < 0.01, ***P < 0.001). For d–f, cells transfected with either shControl or shCaMKII were treated with CQ (20 μM) in the presence or absence of IH (10 μM) for 24 h. d The expressions of p-CaMKII in WCL, and of CaMKII and Drp1 in Mito were examined by Western blot. e The colocalization of CaMKII (red), Drp1 (green), and MitoTracker (blue) was examined by confocal microscopy. Scale bars: 10 μm. f Apoptosis was detected by flow cytometry analysis. The values represent the mean ± SD for three separate experiments (**P < 0.01)

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