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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: The LIM protein Ajuba/SP1 complex forms a feed forward loop to induce SP1 target genes and promote pancreatic cancer cell proliferation

Fig. 4

Depletion of Ajuba dampens SP1 promoted cell proliferation of pancreatic cells. a Western blot assays showed the expression level of Ajuba and SP1 in Patu 8988 cells. b Cell growth rate was detected by CCK-8 assays. c Colony formation assays showed the effect of SP1 and Ajuba on the growth of Patu 8988 cells. Colonies were counted and analyzed after 10 days of post seeding. d Western blot assays showed the expression level of Ajuba and SP1 in PanC1 cells. e Cell growth rate was detected by CCK-8 assays. f Colony formation assays showed the the effect of SP1 and Ajuba on the growth of PanC1 cells. Experiments were repeated twice in triplicates. Data shown was from one reprehensive experiment in triplicates

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