Fig. 5From: Olaparib and ionizing radiation trigger a cooperative DNA-damage repair response that is impaired by depletion of the VRK1 chromatin kinaseQuantification of the effect of VRK1 depletion on the formation of γH2AX and 53BP1 foci induced by olaparib, IR or their combination in HT144 (ATM−/−) cells. a. Effect of si-Control on HT144 cells treated with different doses of olaparib, IR or their combination on the formation of γH2AX foci. b. Effect of si-VRK1 on HT144 cells treated with different doses of olaparib, IR or their combination on the formation of 53BP1 foci in HT144 (ATM−/−) cells. C. Detection of VRK1 depletion in immunoblot. siC: siControl, siV: siVRK1–02. ns: not significant, ** p < 0.01, *** p < 0.001Back to article page