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Table 1 List of antibodies and conditions used in this work

From: Olaparib and ionizing radiation trigger a cooperative DNA-damage repair response that is impaired by depletion of the VRK1 chromatin kinase

Antibody Dilution (WB/IF) Clone and/or reference Supplier
53BP1 Rabbit polyclonal -; 1/200 H300, sc-22,760 Santa Cruz Biotechnology
53BP1 Rabbit polyclonal -; 1/200 NB100–304 Novus Biologicals
Histone H4-K16 ac Rabbit monoclonal 1/500; 1/400 ab109463 Abcam
Nbs1 Mouse monoclonal -; 1/200 611,871 BD Biosciences
VRK1 Mouse monoclonal 1/1000; 1/200 1B5 Own production [74]
VRK1 Rabbit polyclonal 1/1000;- VC Own production [74]
VRK1 (N-term) Rabbit polyclonal -; 1/200 HPA000660 Sigma-Aldrich
β-actin Mouse monoclonal 1/2000; − AC15/A5441 Sigma-Aldrich
γH2AX Mouse monoclonal -; 1/200 Clone JBW301; 05–636 Millipore
Anti-mouse IgG (WB) Goat Anti-Mouse IgG, DyLight 680 (red) 1/10000; − 35,518 Thermo Scientific
Anti-rabbit IgG (WB) Goat Anti-Rabbit IgG, DyLight 800 (green) 1/10000; − 35,571 Thermo Scientific
Goat anti-Mouse IgG (IF) Goat anti-Mouse IgG linked to Cy3 (red) -; 1/1000 115–165-146 Jackson ImmunoResearch; West Grove, PA, USA
Goat anti-rabbit IgG (IF) Goat anti-rabbit IgG linked to Cy2 (green) -; 1/1000 111–225-144 Jackson ImmunoResearch; West Grove, PA, USA