Fig. 3
Oxidative stress is a potent inducer of colonic epithelial CD80. (a) Real-time PCR for CD80 expression in CT26 cell line after treatment with H2O2, 200 μM for 6 h. (b) Flow cytometry was performed for surface expression of CD80 by CT26 cell line after treatment with H2O2, 200 μM for 24 h. Representative histogram plots are shown. (c) Immunofluorescence staining of CD80 in CT26 cells after 24 h treatment with 200 μM H2O2. Magnification: 20X. (d) Flow cytometry analysis of CT26 cells after 1 h of preincubation with 25 mM N-acetyl cysteine and treatment with H2O2 (200 μM) for 24 h. (e) Primary murine colonic epithelial cells were incubated with H2O2, 200 μM for 24 h. Flow cytometry was performed for surface expression of CD80. Representative histogram plots are shown. Mean ± S.E.M. of at least three independent experiments is indicated. *P < 0.05 ** P < 0.01 by unpaired, two-tailed Student’s t-test