Fig. 4

Apoptosis and NF-ĸB signalling are not involved in oxidative stress-mediated CD80 induction. (a) CT26 cells were incubated with H₂O₂ 200 μM for 24 h and assayed for annexin V by flow cytometry. (b) Flow cytometry analysis of CT26 cells after 1 h of preincubation with 25 μM Z-VAD-fmk and treatment with H₂O₂ (200 μM) for 24 h. (c) Immunofluorescence staining of p65 in CT26 cells after 24 h treatment with 10 μM JSH-23, 1 μg/ml LPS or JSH-23 + LPS together. Magnification: × 60. (d) Flow cytometry analysis of CT26 cells after 1 h of preincubation with 10 μM JSH-23 and treatment with H₂O2 (200 μM) for 24 h. Mean ± S.E.M. of at least three independent experiments is indicated. *P < 0.05 ** P < 0.01 by unpaired, two-tailed Student’s t-test