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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Epithelial CD80 promotes immune surveillance of colonic preneoplastic lesions and its expression is increased by oxidative stress through STAT3 in colon cancer cells

Fig. 6

CD80 upregulation is mediated by MAPK activation. (a) Flow cytometry analysis of CT26 cells after 1 h of preincubation with p38 inhibitors (5 μM SB203580 and 5 μM BIRB796) or with JNK inhibitors (10 μM SP600125 and 1 μM AS601245) and treatment with H2O2 (200 μM) for 24 h. Representative histogram plots are shown. (b) Western blotting for phosphoSTAT3 of CT26 cells after treatment with 200 μM H2O2. (c) Representative immunohistochemical staining for phosphoSTAT3 of the colon from AOM treated mice exhibiting activated STAT3 cells in the dysplastic epithelium (100 μm bar). (d) Western blotting for phosphoSTAT3 of CT26 cells after 1 h of preincubation with p38 inhibitor (5 μM SB203580) or with JNK inhibitor (1 μM AS601245) and treatment with H2O2 (200 μM) for 1 h. Mean ± S.E.M. of at least three independent experiments is indicated. *P < 0.05 ** P < 0.01 by unpaired, two-tailed Student’s t-test

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