Fig. 7

CD80 upregulation is mediated by STAT3 activation. CT26 cells were transfected with control or STAT3 siRNAs. After 24 h, cells were treated with 200 μM H₂O₂ for 24 h before qReal Time PCR (a) or flow cytometry (b) for CD80. (c) Flow cytometry analysis of CT26 cells after 1 h of preincubation with 5 μM 5,15-DPP and treatment with H₂O₂ (200 μM) for 24 h. (d) CT26 cells were transfected with plasmid vector alone (CTRL) or to overexpress constitutively active STAT3 (STAT3C); 24 h after transfection, cells were treated with 200 μM H₂O₂ for additional 24 h before flow cytometry for CD80. Flow cytometry analysis of (e) HT-29 and (f) HCT 116 cells after 1 h of preincubation with 5 μM 5,15-DPP and treatment with H2O2 (200 μM) for 24 h. Representative histogram plots are shown for all flow cytometry experiments. Mean ± S.E.M. of at least three independent experiments is indicated. *P < 0.05 ** P < 0.01 by unpaired, two-tailed Student’s t-test