Fig. 6

Acute intraperitoneal injection toxicity test in mice. SPF BALB/c mice were randomly assigned to 7 groups (n = 10, half male and half female mice). The mice were intraperitoneally injected with EE02 once at the indicated concentration (75.00, 86.25, 99.19, 114.19, 131.75 or 151.51 mg/kg b.w.) or injected with DMSO as a control, and the body weight of the mice was assessed every day for two weeks. a The body weights of the female and male mice are shown together. b The body weights of the female mice are shown. c The body weights of the male mice are shown. d Representative photomicrographs show the hematoxylin and eosin (HE)-stained liver, spleen and kidneys of the female mice intraperitoneally injected with EE02 (H&E 200×). e Representative photomicrographs show the HE-stained liver, spleen, kidneys and testes of the male mice intraperitoneally injected with EE02 (H&E 200×)