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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: The regulatory ZFAS1/miR-150/ST6GAL1 crosstalk modulates sialylation of EGFR via PI3K/Akt pathway in T-cell acute lymphoblastic leukemia

Fig. 5

ZFAS1 directly binds miR-150 to regulate ST6GAL1 expression. a Sequence aligment of miR-150 with predicted binding sites in the wild-type and mutant-type regions of ST6GAL1 (left panel) was shown. b The expression of miR-150 was determined in T-ALL cell lines by qRT-PCR. c MiR-150 level was then detected in patients (T-ALL: n = 23, T-ALL/MDR: n = 23). d The correlation between ST6GAL1 and miR-150 was determined using Spearman’s correlation analysis. e, f The ST6GAL1 expression was shown with miR-150 mimic or inhibitor treatment. g Sequence aligment of miR-150 with putative binding sites in sild-type and mutant-type regions of ZFAS1 (left panel) was shown. The wild-type and mutant miRNA target ZFAS1 sequences were fused with luciferace reporter, transfected with miRNA mimic and NC mimic (right panel). h, i ZFAS1 expression was determined in T-ALL cell lines and patients by qRT-PCR (T-ALL: n = 23, T-ALL/MDR: n = 23). j The Spearman’s correlation was used to analyze the relationship between ZFAS1 and miR-150 expression. k, l The ZFAS1 expression was detected by qRT-PCR after cells transfected with miR-150 mimic and inhibitor treatment. m, n The ST6GAL1 level was determined in T-ALL cell lines with different treatment of ZFAS1. o The co-precipitated RNA was detected by qRT-PCR in RNA immunoprecipitation experiment. ZFAS1 and miR-150 were presented as fold enrichment in Ago2 relative to IgG immunoprecipitate. Data were means ± SD of triplicate determinants (*P < 0.05)

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