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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: The regulatory ZFAS1/miR-150/ST6GAL1 crosstalk modulates sialylation of EGFR via PI3K/Akt pathway in T-cell acute lymphoblastic leukemia

Fig. 6

The reversal effect of ZFAS1/miR-150 co-expression on T-ALL chemoresistance (a) ST6GAL1 expression was detected by qRT-PCR and western blot with co-transfection siZFAS1 and anti-miR-150 in T-ALL cell lines. b The salic acid levels (FITC-SNA) of different treated T-ALL cell lines were shown by FCM. c, d The proliferative ability of different cells was measured by CCK-8 assay and colony formation analysis. e Ki67 expression was showed by immunoflourence staining. f The viability of T-ALL cells was detected by CCK8 with ADR treatment. g The altered IC50 values were calculated. h Flow cytometry showed the apoptosis rate of transfected cells in response to ADR. i qRT-PCR and western blot were carried out to detect ST6GAL1 expression in co-transfection of ZFAS1 and miR-150 in T-ALL cell lines. j FITC-SNA was detected by FCM to show the salid acid level of transfected T-ALL cell surface. k The proliferative ability was detected using CCK-8 assay at 0, 24, 48, 72, 96 h. l, m Colony formation analysis and Ki67 staining were performed to detect proliferative ability. n The cell viability was determined by CCK-8 assay. o The IC50 values of transfected T-ALL cell lines were calculated. p The apoptosis induced by ADR was determined by FCM. Data were means ± SD of triplicate determinants (*P < 0.05)

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