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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Exosomes from the tumour-adipocyte interplay stimulate beige/brown differentiation and reprogram metabolism in stromal adipocytes to promote tumour progression

Fig. 4

Breast cancer cells undergo metabolic adaptation in the presence of adipocytes. a Left, tumour cells cocultivated with or without mature adipocytes were stained with red oil. Right, the TG content in tumour cells. b Glucose uptake was evaluated over time by 2-NBDG (50 μM) in glucose-free DMEM without and with insulin (INS) (50 μg/ml) or apigenin (Api) (50 μM), and the results are expressed as the mean fluorescence intensity (MFI). AD: adipocytes. c Raw data for the OCR and ECAR as determined by the Seahorse XF24 analyser. The ECAR was evaluated after the addition of 10 mM glucose to tumour cells in the presence or absence of adipocytes. The OCR was measured in the presence of palmitate as described in the Methods. d MDA-MB-231 cells were cocultivated in the presence or absence of adipocytes. After 3 days, protein was extracted for western blot analysis of the expression of the indicated proteins, and (e) mRNA was extracted for the qPCR analysis of the expression of the indicated genes. Data are presented as the mean ± S.D. of at least three independent experiments. * P < 0.05 versus control values

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