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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Exosomes from the tumour-adipocyte interplay stimulate beige/brown differentiation and reprogram metabolism in stromal adipocytes to promote tumour progression

Fig. 6

miRNA-144 suppresses the beige/brown differentiation of adipocytes by downregulating MAP3K8, and miRNA-126 remodels adipocyte metabolism by downregulating IRS-1. The adipocytes in the cytochalasin D (CytoD) group were treated with CytoD (final concentration, 2 μg/ml) and 20 μg of exosomes purified from cancer-associated adipocyte conditioned medium (CA-CM). a The predicted miRNA-144 binding site in the 3’UTR of the MAP3K8 gene and the predicted miRNA-126 binding site in the 3’UTR of the IRS-1 gene from TargetScan. b The GV272 vector containing the 3’UTR of the target gene harbouring wild-type (wt) or mutated (mt) miRNA binding sites was transfected into HEK 293 T cells stably expressing miRNA or empty vector (as a control). Luciferase activity was analysed at 48 h post-transfection, and the ratio of firefly luciferase activity to Renilla luciferase activity is shown. c Breast cancer cells were transfected with miRNA-126 inhibitor, mature adipocytes were transfected with miRNA-126 mimic as the positive control and the control vector was applied as the negative control. Mature adipocytes cultured in the presence or absence of tumour cells for 3 days were stained with red oil (d), and mRNAs were extracted for the qPCR analysis of the expression of the indicated genes. e, f Western blot analysis of related protein expression in different groups. Data are presented as the mean ± S.D. of at least three independent experiments. * P < 0.05 versus control values

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